P2 Laboratory
P2 Laboratory
Catalog
What is the P2 Lab
P2 laboratory requirements
1, the main technical indicators to determine
Two 2, building, structure and decoration requirements
Three 3, air conditioning, ventilation and purification
Four 4, drainage
Five 5, electrical settings
Six 6 Fire
Seven 7, should be equipped with the process equipment
P2 laboratory sample room
Open
What is the P2 Lab
P2 laboratory requirements
1, the main technical indicators to determine
Two 2, building, structure and decoration requirements
Three 3, air conditioning, ventilation and purification
Four 4, drainage
Five 5, electrical settings
Six 6 Fire
Seven 7, should be equipped with the process equipment
P2 laboratory sample room
Open
Edit this section what is the P2 Laboratory
P2 laboratory refers to the two level of biological safety laboratory, also known as BSL-2. Biological laboratory safety level
A classification of. Among all kinds of laboratories, P2 lab is the most widely used biological safety laboratory. Its grades are P1, P2, P3, P4. In accordance with the requirements of the nature of the laboratory to choose the appropriate level. WHO (WHO) in accordance with the risk of disease and infection, etc.,
The infectious organisms were divided into 4 types. According to the equipment and technical conditions, the biological
Laboratory is also divided into 4 levels (generally referred to as P1, P2, P3, P4 laboratory), the 1 most
Low, 4 highest. P1 laboratory is generally applicable to healthy adults without pathogenic effect
P2 is suitable for microorganisms which have moderate potential hazards to human and environment;
P3 is applied mainly through the respiratory pathway to make people infected with serious or even fatal diseases.
Pathogenic microorganisms or toxins of the disease; P4 applicable to the human body with a high degree of risk
There is no clear way to spread or propagate through the sol route, and there is no effective disease at present.
Pathogenic microorganisms or their toxins in the seedling or treatment method.
Edit this section P2 laboratory requirements
First, the determination of main technical indicators
P2 is mainly used for laboratory diagnosis and research of primary health services, and the damage grade subjects for grade II (individual secondary hazards, hazard, finite population) specifically defined as "can cause the disease of human or animal, but the general situation of health workers, community, livestock or the environment will not cause serious harm to the pathogen. Laboratory infection does not lead to serious disease, with effective treatment and prevention measures, and the spread of risk is limited". According to the above, the national standard of the national standard for the construction technology of the biological safety laboratory provides the following technical indicators (static) for the BLS-2 laboratory:
1, cleanliness: no requirement
2, minimum ventilation frequency: can open the window ventilation
3, and the adjacent room adjacent to the pressure difference: no requirement
4, temperature: 18~27
5, relative humidity%:30~70
6, noise dB (A): less than 60
7, minimum illumination lx:300
* appeal target is to establish the minimum requirements of the P2 laboratory, the builder can be based on the actual needs
Appropriate to improve the corresponding indicators, such as cleanliness, pressure etc..
Two, building, structure and decoration requirements
Now BLS-2 is rebuilt in most laboratories in existing buildings, different from the new P2 Laboratory (GB, new P2 labs should be away from the public place), between in and adjacent to the other room, there is a relatively isolated problem. Therefore, in the original building alterations P2 laboratory, should pay attention to the following issues.
1, in the sharing of buildings in the building P2 laboratory, should be set up to automatically close the door with a lock, when necessary, can be set up in the buffer area, such as buffer between.
2, if there is no mechanical ventilation system, should be the window for natural ventilation, and have the insect screens. (in general, there should be a mechanical ventilation system, otherwise, it is difficult to ensure the temperature and humidity).
3, should have anti insects, rats and other animals to enter and escape measures.
Three, air conditioning, ventilation and purification
In order to meet the requirements of P2 laboratory temperature and humidity, it is suitable to be equipped with mechanical ventilation system, there are special requirements of the design requirements can be added, such as additional purification requirements and according to the purification requirements for the return (exhaust) wind system design, etc.. In this regard, we should focus on the following issues:
1, can use the air conditioning system with circulating wind. If it involves chemical solvent, infectious material operation and animal experiment, it should adopt full exhaust system.
2, biological safety cabinet and exhaust system connection
I level exhaust ratio 100%, closed connection.
II class A type exhaust ratio of 30%, can be discharged into the room or set local exhaust hood.
B1 type exhaust fan ratio 70%, closed connection.
B2 type exhaust fan ratio 100%, closed connection.
3, all kinds of equipment in the laboratory should be conducive to the position of the airflow from the "clean" space to the "pollution" of space flow, minimize indoor backflow and leakage (biological safety cabinets should generally be placed in indoor air is the most downstream, away from the delivery outlet).
* the exhaust air of the biological safety cabinet can not be replaced by the indoor exhaust air.
Four, drainage
Waste water containing bacteria should be discharged to the municipal wastewater discharge system after high temperature and high pressure disinfection and chemical disinfection
Five, electrical settings
1, should be set up special distribution box.
2, the power should be set to the leakage detection alarm device.
3, there should be a reliable grounding system, the grounding resistance should not be greater than 1.
4, the laboratory entrance should be the state of the work of the text type light signal display.
5, should be set up emergency evacuation signs.
Six, fire
The refractory grade of the building is not less than two
Seven, should be equipped with the process equipment
1, biological safety cabinet
(1) to produce microbial aerosol or from the operation, you can use the I biological safety cabinet.
Biological safety cabinet
(2) treatment of emotional materials, should use II class biological safety cabinets;
(3) the treatment of chemical carcinogens, radioactive substances and volatile solvent, should use the -B class II biological safety cabinet.
2, high temperature and high pressure sterilization cabinet.
3, eye wash, when necessary, should be set up emergency spray device.
* ultra clean bench is not a biological safety cabinet, can not be used for biological safety operation.
P2 laboratory is used for preliminary biological safety experiments, the national standard clear the corresponding requirements. The establishment of P2 laboratory should be based on the national standard, in ensuring the safety of the premise to determine the construction objectives, the establishment of the laboratory should be safe, economic laboratory.
Edit this section of the P2 laboratory model room
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